Research Unit Proteolysis
in the Golgi Apparatus
Dr. Matthias Voss
+49 431 880-1676
Research in the Voss lab aims to elucidate how proteolytic processing of glycosylation enzymes in the Golgi apparatus contributes to or is interconnected with the overall organisation of the Golgi. Combining tailored engineered cell culture models, protein biochemistry and cell biology tools as well as state-of-the-art proteomics, the lab studies in particular the intramembrane protease SPPL3, which has emerged as a potent physiological regulator of glycosylation.
Publications
2025
Regulation of podocyte surface proteins by the enzyme A Disintegrin And Metalloproteinase 10 (ADAM10).
Rosenbaum D, Reichelt J, Gudaitis S, Kühne S, Zielinski S, Loreth D, Blume L, Brand J, Vitzthum H, Sachs W, Lampert A, Seipold L, Voss M, Meyer-Schwesinger C, Saftig P.
Kidney Int. 2025 May 6:S0085-2538(25)00344-8. Epub ahead of print.
2024
GOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway.
Brauer BK, Chen Z, Beirow F, Li J, Meisinger D, Capriotti E, Schweizer M, Wagner L, Wienberg J, Hobohm L, Blume L, Qiao W, Narimatsu Y, Carette JE, Clausen H, Winter D, Braulke T, Jabs S, Voss M.
EMBO J. 2024 Dec;43(24):6264-6290.
Proteolytic cleavage of Golgi glycosyltransferases by SPPL3 and other proteases and its implications for cellular glycosylation.
Voss M.
Biochim Biophys Acta Gen Subj. 2024 Jul;291(13):2733-2766.
Cellular depletion of major cathepsin proteases reveals their concerted activities for lysosomal proteolysis.
Gallwitz L, Bleibaum F, Voss M, Schweizer M, Spengler K, Winter D, Zöphel F, Müller S, Lichtenthaler S, Damme M, Saftig P.
Cell Mol Life Sci. 2024 May 22;81(1):227.
Functional genomics identifies N-acetyllactosamine extension of complex N-glycans as a mechanism to evade lysis by natural killer cells.
Zhuang X, Woods J, Ji Y, Scheich S, Mo F, Rajagopalan S, Coulibaly ZA, Voss M, Urlaub H, Staudt LM, Pan KT, Long EO.
Cell Rep. 2024 Apr 14;43(4):114105.
2023
EGFR stimulation enables IL-6 trans-signalling via iRhom2-dependent ADAM17 activation in mammary epithelial cells
Schumacher N, Thomsen I, Brundert F, Hejret V, Düsterhöft S, Tichý B, Schmidt-Arras D, Voss M, Rose-John S.
Biochim Biophys Acta Mol Cell Res. 2023 October; 1870(7):119489.
Viral host range factors antagonize pathogenic SAMD9 and SAMD9L variants
Gahr S, Perinetti Casoni G, Falk-Paulsen M, Maschkowitz G, Bryceson YT, Voss M.
Exp Cell Res. 2023 Apr 15;425(2):113541.
2022
Endogenous tagging reveals a mid-Golgi localization of the glycosyltransferase-cleaving intramembrane protease SPPL3
Truberg J, Hobohm L, Jochimsen A, Desel C, Schweizer M, Voss M.
Biochim Biophys Acta Mol Cell Res. 2022 Nov;1869(11):119345.
N- terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion.
Hobohm L, Koudelka T, Bahr FH, Truberg J, Kapell S, Schacht SS, Meisinger D, Mengel M, Jochimsen A, Hofmann A, Heintz L, Tholey A, Voss M.
Cell Mol Life Sci. 2022 Mar 13;79(3):185
Inhibition of ADAM17 impairs endothelial cell necroptosis and blocks metastasis.
Bolik J, Krause F, Stevanovic M, Gandraß M, Thomsen I, Schacht SS, Rieser E, Müller M, Schumacher N, Fritsch J, Wichert R, Galun E, Bergmann J, Röder C, Schafmayer C, Egberts JH, Becker-Pauly C, Saftig P, Lucius R, Schneider-Brachert W, Barikbin R, Adam D, Voss M, Hitzl W, Krüger A, Strilic B, Sagi I, Walczak H, Rose-John S, Schmidt-Arras D.
J Exp Med. 2022 Jan 3;219(1):e20201039
2020
Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets.
Galgano D, Soheili T, Voss M, Torralba-Raga L, Tesi B, Cichocki F, Andre I, Rettig J, Cavazzana M, Bryceson Y.
Front Immunol. 2020 Jun 9;11:1154
2018
A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation.
Tesi B, Rascon J, Chiang SCC, Burnyte B, Löfstedt A, Fasth A, Heizmann M, Juozapaite S, Kiudeliene R, Kvedaraite E, Miseviciene V, Muleviciene A, Müller ML, Nordenskjöld M, Matuzeviciene R, Samaitiene R, Speckmann C, Stankeviciene S, Zekas V, Voss M, Ehl S, Vaiciene-Magistris N, Henter JI, Meeths M, Bryceson YT.
J Allergy Clin Immunol. 2018 Jul;142(1):317-321.e8
Constitutional SAMD9L mutations cause familial myelodysplastic syndrome and transient monosomy 7.
Pastor VB, Sahoo SS, Boklan J, Schwabe GC, Saribeyoglu E, Strahm B, Lebrecht D, Voss M, Bryceson YT, Erlacher M, Ehninger G, Niewisch M, Schlegelberger B, Baumann I, Achermann JC, Shimamura A, Hochrein J, Tedgård U, Nilsson L, Hasle H, Boerries M, Busch H, Niemeyer CM, Wlodarski MW.
Haematologica. 2018 Mar;103(3):427-437
2017
Gain-of-function SAMD9L mutations cause a syndrome of cytopenia, immunodeficiency, MDS, and neurological symptoms.
Tesi B*, Davidsson J*, Voss M*, Rahikkala E, Holmes TD, Chiang SCC, Komulainen-Ebrahim J, Gorcenco S, Rundberg Nilsson A, Ripperger T, Kokkonen H, Bryder D, Fioretos T, Henter JI, Möttönen M, Niinimäki R, Nilsson L, Pronk CJ, Puschmann A, Qian H, Uusimaa J, Moilanen J, Tedgård U, Cammenga J, Bryceson YT.
Blood. 2017 Apr 20;129(16):2266-2279
Natural killer cell biology illuminated by primary immunodeficiency syndromes in humans.
Voss M, Bryceson YT.
Clin Immunol. 2017 Apr;177:29-42
2016
Proteolytic Processing of Neuregulin 1 Type III by Three Intramembrane-cleaving Proteases.
Fleck D, Voss M, Brankatschk B, Giudici C, Hampel H, Schwenk B, Edbauer D, Fukumori A, Steiner H, Kremmer E, Haug-Kröper M, Rossner MJ, Fluhrer R, Willem M, Haass C.
J Biol Chem. 2016 Jan 1;291(1):318-33.
2015
Secretome analysis identifies novel signal Peptide peptidase-like 3 (Sppl3) substrates and reveals a role of Sppl3 in multiple Golgi glycosylation pathways.
Kuhn PH*, Voss M*, Haug-Kröper M, Schröder B, Schepers U, Bräse S, Haass C, Lichtenthaler SF, Fluhrer R.
Mol Cell Proteomics. 2015 Jun;14(6):1584-98
2014
Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation.
Voss M, Künzel U, Higel F, Kuhn PH, Colombo A, Fukumori A, Haug-Kröper M, Klier B, Grammer G, Seidl A, Schröder B, Obst R, Steiner H, Lichtenthaler SF, Haass C, Fluhrer R.
EMBO J. 2014 Dec 17;33(24):2890-905
Differential protein-protein interactions of full length human FasL and FasL fragments generated by proteolysis.
Lettau M*, Voss M*, Ebsen H, Kabelitz D, Janssen O.
Exp Cell Res. 2014 Jan 15;320(2):290-301
2013
Mechanism, specificity, and physiology of signal peptide peptidase (SPP) and SPP-like proteases.
Voss M, Schröder B, Fluhrer R.
Biochim Biophys Acta. 2013 Dec;1828(12):2828-39. Review
2012
Foamy virus envelope protein is a substrate for signal peptide peptidase-like 3 (SPPL3).
Voss M, Fukumori A, Kuhn PH, Künzel U, Klier B, Grammer G, Haug-Kröper M, Kremmer E, Lichtenthaler SF, Steiner H, Schröder B, Haass C, Fluhrer R.
J Biol Chem. 2012 Dec 21;287(52):43401-9
2010
Requirements for leukocyte transmigration via the transmembrane chemokine CX3CL1.
Schwarz N, Pruessmeyer J, Hess FM, Dreymueller D, Pantaler E, Koelsch A, Windoffer R, Voss M, Sarabi A, Weber C, Sechi AS, Uhlig S, Ludwig A.
Cell Mol Life Sci. 2010 Dec;67(24):4233-48
The adapter protein Nck: role of individual SH3 and SH2 binding modules for protein interactions in T lymphocytes.
Lettau M, Pieper J, Gerneth A, Lengl-Janssen B, Voss M, Linkermann A, Schmidt H, Gelhaus C, Leippe M, Kabelitz D, Janssen O.
Protein Sci. 2010 Apr;19(4):658-69
2009
Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening.
Voss M*, Lettau M*, Janssen O.
BMC Immunol. 2009 Oct 6;10:53
2008
Posttranslational regulation of Fas ligand function.
Voss M*, Lettau M*, Paulsen M, Janssen O.
Cell Commun Signal. 2008 Dec 29;6:11